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0_equals_true
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27 Feb 2009, 12:32 pm

If use Surgical Sprit for DNA ethyl precipitation, will this be a problem because it also contain methanol?



lelia
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27 Feb 2009, 12:52 pm

Hmm. I'm trying to call up a mental image of the bottle of alcohol used. White, rectangular, but I can't remember the label. It's been over 35 years since I precipitated DNA.



Woodpecker
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27 Feb 2009, 4:12 pm

I have never worked with DNA, but I guess that if it is not damaged by ethanol than a trace of methanol in the ethanol should not matter.

It will be best if you look around WP for a DNA expert or ask LabPet.

She knows a lot more than me about that type of thing.


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Health is a state of physical, mental and social wellbeing and not merely the absence of disease or infirmity :alien: I am not a jigsaw, I am a free man !

Diagnosed under the DSM5 rules with autism spectrum disorder, under DSM4 psychologist said would have been AS (299.80) but I suspect that I am somewhere between 299.80 and 299.00 (Autism) under DSM4.